Tibet Development and Breeding Application of the Fluorescence Molecular Marker of Rice Bacterial Blight Resistance Gene <I>Xa7</i>
نویسندگان
چکیده
In this study, the closely linked fluorescence molecular marker PM-Xa7 of Xa7 gene was developed on basis of sequence difference between resistant variety ‘IRBB7’ containing and other nine bacterial blight susceptible rice varieties, and combine with PARMS technology (Penta-primer amplification refractory mutation system). Genotypes were identified in 72 germplasm resistance level ‘Mei B’ improved by backcross screening marker. The results showed that two germplasms screened out from germplasm; By introducing resistant into PM-Xa7, a stable new maintainer to successfully obtained. signal detected 289 plants 93 BC 2 F population generated B’, genotypes were consistent phenotypes Xoo inoculation. These research indicated can be effectively used for marker-assisted breeding gene.
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ژورنال
عنوان ژورنال: Molecular plant breeding
سال: 2021
ISSN: ['1923-8266']
DOI: https://doi.org/10.5376/mpb.2021.12.0023